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Liu, Zhe ; Lebrun, Vincent ; Kitanosono, Taku ; Mallin, Hendrik ; Köhler, Valentin ; Häussinger, Daniel ; Hilvert, Donald ; Kobayashi, Shu ; Ward, Thomas R. ( , Angewandte Chemie)more » « less
Abstract Regulation of enzymatic activity is vital to living organisms. Here, we report the development and the genetic optimization of an artificial zymogen requiring the action of a natural protease to upregulate its latent asymmetric transfer hydrogenase activity.
